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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which ends up in chromosome instability, and thus may perhaps add to tumorigenesis. This protein was identified to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a critical function in selling cell-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two further splice variants were described but thorough nucleotide sequence info is not really nevertheless accessible.
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Blocking peptides are peptides that bind particularly to the concentrate on antibody and block antibody binding. These peptide normally consists of the epitope recognized from the antibody.
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This information outlines further more feasible optimization of cellular and immunoassay parameters to make sure the best possible final results are...
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This information outlines even more possible optimization of cellular and immunoassay parameters to guarantee the absolute best effects are...
Antibodies certain to the blocking peptide no longer bind to the epitope to the goal protein. By comparing the staining with the blocked antibody compared to the antibody on your own, one can see which staining is particular.
Goal Data Cyclin E belongs on the hugely conserved cyclin family, whose associates are characterized by a remarkable periodicity in protein abundance throughout the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins show unique expression and degradation patterns which add to the temporal coordination of each and every mitotic event. Cyclin E kinds a complex with and features being a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development through S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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